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KMID : 0545120150250030393
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Journal of Microbiology and Biotechnology
2015 Volume.25 No. 3 p.393 ~ p.398
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Screening and Development of DNA Aptamers Specific to Several Oral Pathogens
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Park Jung-Pyo
Shin Hye-Joo
Park Suk-Gyun
Oh Hee-Kyun
Choi Choong-Ho
Park Hong-Ju
Kook Min-Suk
Ohk Seung-Ho
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Abstract
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Aptamers are composed of single-stranded oilgonucleotides that can selectively bind desired molecules. It has been reported that RNA or DNA could act as not only a genetic messenger but also a catalyst in metabolic pathways. RNA aptamers (average sizes 40-50 bp) are smaller than antibodies and have strong binding capacities to target molecules, similar to antigenantibody interactions. Once an aptamer was selected, it can be readily produced in large quantities at low cost. The objectives of this study are to screen and develop aptamers specific to oral pathogens such as Porphyromonas gingivalis, Treponema denticola, and Streptococcus mutans. The bacterial cell pellet was fixed with formaldehyde as a target molecule for the screening of aptamers. The SELEX method was used for the screening of aptamers and a modified western blot analysis was used to verify their specificities. Through SELEX, 40 kinds of aptamers were selected and the specificity of the aptamers to the bacterial cells was confirmed by modified western blot analysis. Through the SELEX method, 40 aptamers that specifically bind to oral pathogens were screened and isolated. The aptamers showed possibility as effective candidates for the detection agents of oral infections.
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KEYWORD
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Aptamer, Porphyromonas gingivalis, Treponema denticola, Streptococcus mutans, Streptococcus oralis, Streptococcus sanguis
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